FLIM is a form of photon-counting imaging and is now a well-established imaging technique in which an image is composed of individual photon emissions whose position is recorded during the detection process. The result of fluorescence lifetime microscopy is thus images of the sample in which each image pixel represents the fluorescence lifetime with respect to its corresponding position. Besides applications in chemistry and materials research, FLIM is mainly used in molecular biophysics, i.e. for the study of biological processes and materials. Medical research in particular benefits from the ever-improving imaging capabilities at the molecular level.
In case you would like to learn more about data acquisition in fluorescence lifetime imaging, we recommend reading our detailed article on this fascinating topic.